ELISA DIS3-like exonuclease 2 (DIS3L2)

Reactivity: (Homo sapiens) UniProt:Q8IYB7 Abbreviation:DIS3L2 Alternative Names:FAM6A; FLJ36974; MGC42174; OTTHUMP00000203808|family with sequence similarity 6; member A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.8% Inter-AssayCV:?8.3% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DIS3L2 in samples. An antibody specific for DIS3L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIS3L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DIS3L2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIS3L2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The exosome is a mµLtisubunit complex composed of several highly conserved subunits, some of which are 3' to 5' exoribonucleases. The complex is involved in a variety of cellµLar processes and is responsible for degrading unstable mRNAs that contain AU-rich (ARE) elements in their untranslated 3' region. DIS3L2 (DIS3-like exonuclease 2) is an 885 amino acid protein that is thoµght to function as an exonuclease and may be required for the 3' processing of pre-mRNA into mature mRNA. Defects or chromosomal translocations involving the gene encoding DIS3L2 may be associated with Marfanoid habitus, a genetic disorder characterized by abnormalities in the skeleton, eyes and cardiovascµLar system. DIS3L2 is expressed as five isoforms due to alternative splicing events. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).