hLH Ovulation test

hLH Ovulation test - The Diagnostik Nord hLH Ovulation test is a rapid chromatographic immunoassay for the qualitative detection of elevated levels of human luteinizing hormone (hLH) in urine to aid in the detection of ovulation and the determination of the most fertile days of the female cycle. SUMMARY: Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. In order for pregnancy to occur, the egg must be fertilized by sperm within 24 hours after its release. Immediately prior to ovulation, the body produces a large amount of luteinizing hormone (LH) which triggers the release of a ripened egg from the ovary. This “LH surge” usually takes place in the middle of the menstrual cycle.1 The Diagnostik Nord hLH Ovulation test is a complete system to help predict the time of ovulation, and peak fertility. It is during this fertile time that pregnancy is most likely to occur. The Diagnostik Nord hLH Ovulation test detects the LH surge in urine, signaling that ovulation is likely to occur in the next 24-36 hours. The test utilizes a combination of antibodies including a monoclonal LH antibody to selectively detect elevated levels of LH. NOTE: The LH surge and ovulation may not occur in all cycles. REAGENTS: The test contains anti-LH particles and anti-LH antibody coated on the membrane. SPECIMEN COLLECTION AND PREPARATION Determine the optimal time for urine collection. For best results, collect urine at about the same time each day. Some women have found that their best specimen is after 12 noon. Do not collect the first urine specimen after waking up. Reduce liquid intake approximately 2 hours prior to urine collection. Record the date, cycle day and time of urine collection. See the TEST RESULTS CHART at the end of this package insert. Urine can be stored at room temperature for up to 8 hours or at 2-8 °C for up to 24 hours. Do not freeze. For best results, test urine on the same day that it is collected. If refrigerated, let urine reach room temperature before testing. Do not shake the container. If a sediment forms at the bottom of the collection container, allow the sediment to settle. Use only urine from the top of the container. Materials Provided Diagnostik Nord hLH ovulation test cards Disposable droppers Package insert WHEN TO START TESTING: First, determine the indivudual’s Menstrual Cycle Length. The Menstrual Cycle Length is the number of days from the first day of the subject’s period (menstrual bleeding) to the last day before the next period starts. Next, determine the Days to Count Ahead after the period to start testing. Find the individual’s Menstrual Cycle Length on the first row of the chart below, and read the corresponding Days to Start in the second row. This is the number of days after the period to begin testing. Finally, determine the day to start testing. Starting from and including the first day of the last period, count ahead the number of days indicated in the previous step. This is the day on which testing should begin. As a basic guideline, it is recommended to test once a day for five days. Note: If uncertain about the length of the individual’s menstrual cycle, use the shortest menstrual cycle length (21 days) when reading the chart. In this case, it may be necessary to test for more than 5 days. Example: The subject’s usual menstrual cycle length is 28 days. The chart indicates to count ahead 11 days from the subject’s last period. The subject’s last period started on the 3rd. Starting from and including the 3rd, count ahead 11 days to arrive at the 13th. Urine collection and testing should start on the 13th and proceed through the 17th. INTERPRETATION OF RESULTS: Negative: The test line (T) is less intense than the control line (C) or the test line (T) does not appear. This indicates that no LH surge has been detected. Positive: Two lines are visible, and the line in test line region (T) is the same as or darker than the one in the control line region (C). This indicates that ovulation will probably take place in the next 24-36 hours. Invalid Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your distributor.