pMEV2HA-AKT1-DD

The pMEV2HA-AKT1-DD plasmid expresses a constitutively active form of human AKT1, with dual aspartic acid mutations at Thr308 and Ser473 (T308D/S473D) that mimic phosphorylation and sustain continuous kinase activity without upstream stimulation. Cloned into the pMEV2HA vector, this construct features an N-terminal HA-tag for easy detection in mammalian cells. Ideal for studying PI3K/AKT signaling pathways, cell survival, metabolism, and cancer-related processes, pMEV2HA-AKT1-DD enables researchers to bypass growth factor activation and directly assess downstream effects of active AKT1. This Xpress Clone is sequence-verified and ready for high-efficiency transfection and expression in various mammalian systems. - 20 ug